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| Wojnicz,Dorota; Tichaczek-Goska,Dorota. |
| The aim of this study was to determine the effect of subinhibitory concentrations (sub-MICs) of ciprofloxacin, amikacin and colistin on biofilm formation, motility, curli fimbriae formation by planktonic and biofilm cells of E. coli strains isolated from the urine of patients with various urinary system infections. Quantification of biofilm formation was carried out using a microtiter plate assay and a spectrophotometric method. Bacterial enumeration was used to assess the viability of bacteria in the biofilm. Curli expression was determined by using YESCA agar supplemented with congo red. Using motility agar the ability to move was examined. All the antibiotics used at sub-MICs reduced biofilm formation in vitro, decreased the survival of bacteria, but... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilm; Sub-MIC; Ciprofloxacin; Amikacin; Colistin. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100037 |
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| Mohamed,Yasmine Fathy; Abou-Shleib,Hamida Moustafa; Khalil,Amal Mohamed; El-Guink,Nadia Mohamed; El-Nakeeb,Moustafa Ahmed. |
| Abstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in this study to demonstrate the membrane permeabilizing activity of colistin focusing on investigating its efficiency toward those pan-drug resistant isolates which represent a critical situation. We determined the killing dynamics of colistin against pan-drug resistant isolates. The permeability alteration was confirmed by different techniques as: leakage, electron microscopy and construction of an artificial membrane model; liposomes. Moreover, selectivity of colistin against microbial cells was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pan-drug resistant; Leakage; Permeability alteration; Colistin; Ultrastructure. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200381 |
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